Protein targeting into mitochondria in tobacco BY-2 cell visualized by
fluorescent protein (GFP). Red color: fluorescence of mitochondrial-specific dye.
Yellow color: merged image of fluorescences from GFP and the mitochondrial-specific
Scale bar = 50um. (This work is the collaboration with Lab. Plant Mol. Genet., Univ. Tokyo
and Lab. Mol. Biodiversity, Natl. Inst. Agrobiol. Sci.)
Lab. Cell and Genome Biol.
Plant Organelles Analysis Group
Nakao KUBO, Ph.D.
Graduate School of Life and
Kyoto Prefectural University
Kyoto Pref. Inst. Agr. Biotech.,
74 Oji, Kitainayazuma, Seika,
Soraku 619-0244, JAPAN
As you may know, "MITOCHONDRION" is one of the cellular organelles,
main role is production of energy by oxidative phosphorylation. According
endosymbiont hypothesis, this organelle is thought to be derived from some
bacteria which were entered into the host cell.
Current mitochondria have original genome, so-called "mitochondrial
"mitochondrial DNA". However, there are limited number of genes and most
mitochondrial proteins are encoded by the nuclear genes. It has been proposed
these nuclear-encoded mitochondrial genes have been transferred from the
mitochondria to the nucleus during evolution. In general, the nuclear-encoded
genes have targeting signals which are involved in protein import to the
compartment. However, the origin and the mechanism of acquisition of these
targeting signals are not clearly understood.
In order to elucidate this question, we have been searching the nuclear
had been transferred from the mitochondrion to the nucleus in relatively recent past.
To date, we have isolated a number of genes from the nuclear genomes in
plants, determined the origin of the targeting signals, and proposed mechanisms
explain their acquisition.
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Since Nov. 2003
Last updated Apr. 6 2017